Cell Culture:Article Title: Tyrosine tRNA synthetase as a novel extracellular immunomodulatory protein in Streptococcus anginosus.
Article Snippet: Streptococcus anginosus is frequently detected in patients with infective endocarditis, abscesses or oral cancer.. Although S. anginosus is considered the causative pathogen of these diseases, the pathogenic mechanisms of the bacterium have remained unclear.. Previously, we suggested that an extracellular antigen from S. anginosus (SAA) serves as a pathogenic factor by inducing nitric oxide production in murine macrophages.
In Vitro:
Article Title: <p>Potential Allylpyrocatechol Derivatives as Antibacterial Agent Against Oral Pathogen of<em> S. sanguinis</em> ATCC 10,556 and as Inhibitor of MurA Enzymes: in vitro and in silico Study</p>
Article Snippet: .. S. sanguinis ATCC 10,556 and as Inhibitor of MurA Enzymes: in vitro and in silico Study This article was published in the following Dove Press journal: Drug Design, Development and Therapy Dikdik Kurnia Geofanny Sarah Hutabarat Devi Windaryanti Tati Herlina Yetty Herdiyati Mieke Hemiawati Satari 1Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Sumedang, Indonesia; 2Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Indonesia; 3Department of Oral Biology, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Indonesia Background: Streptococcus sanguinis is Gram-positive bacteria that contribute to caries. ..
In Silico:
Article Title: <p>Potential Allylpyrocatechol Derivatives as Antibacterial Agent Against Oral Pathogen of<em> S. sanguinis</em> ATCC 10,556 and as Inhibitor of MurA Enzymes: in vitro and in silico Study</p>
Article Snippet: .. S. sanguinis ATCC 10,556 and as Inhibitor of MurA Enzymes: in vitro and in silico Study This article was published in the following Dove Press journal: Drug Design, Development and Therapy Dikdik Kurnia Geofanny Sarah Hutabarat Devi Windaryanti Tati Herlina Yetty Herdiyati Mieke Hemiawati Satari 1Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Sumedang, Indonesia; 2Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Indonesia; 3Department of Oral Biology, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Indonesia Background: Streptococcus sanguinis is Gram-positive bacteria that contribute to caries. ..
Activity Assay:Article Title: Lactic acid bacteria isolated from fermented flour of finger millet, its probiotic attributes and bioactive properties
Article Snippet: .. Tanaka et al. (1999) stated that BSH activity is high in genus Bifidobacterium compared to Lactobacillus and further suggested that high BSH activity is commonly present in strains isolated from gastrointestinal track of mammals, when Table 8 Anti-bacterial activity of intracellular cell free extracts of isolated lactic acid bacteria Test pathogens Mean diameter of the zones of inhibition (in mm) R17 RV02 RV19 RV28 O24 E. coli ATCC 2592 15.33 ± 0.33b 16.67 ± 1.45b 15.33 ± 0.33b 17.33 ± 0.33b 15.33 ± 0.33b K. pneumonia ATCC 35594 15.33 ± 0.33c 14.66 ± 0.33c 15.00 ± 0.00c 18.33 ± 0.33b 18.33 ± 0.33b S. aureus ATCC 6571 10.33 ± 0.33d 13.33 ± 0.33c 12.66 ± 0.33c 13.33 ± 0.33c 13.00 ± 0.57c S. sanguinis ATCC 10556 0.00 ± 0.00b 0.00 ± 0.00b 0.00 ± 0.00b 0.00 ± 0.00b 0.00 ± 0.00b S. salvarius ATCC 13419 14.66 ± 0.33d 16.00 ± 0.00c 16.00 ± 0.00c 10.66 ± 0.33e 14.00 ± 0.00d S. flexenari ATCC 12022 14.33 ± 0.33b 15.66 ± 0.33b 16.00 ± 0.00b 12.33 ± 0.66c 15.66 ± 0.33b E. faecilis ATCC 49532 8.33 ± 0.33d 0.00 ± 0.00e 0.00 ± 0.00e 0.00 ± 0.00c 12.66 ± 0.33e A. baumani ATCC 17978 0.00 ± 0.00g 15.33 ± 0.33c 11.33 ± 0.33f 13.00 ± 0.00e 14.33 ± 0.33d S. mutans ATCC 25175 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c S. pyogenes ATCC 700294 0.00 ± 0.00e 12.00 ± 0.00c 12.33 ± 0.33c 15.00 ± 0.57b 13.66 ± 0.33b,c E. coli ATCC35218a 15.33 ± 0.33c 16.00 ± 0.00c 18.33 ± 0.33b 16.66 ± 0.33b,c 15.33 ± 0.33c S. aureusa NCTC13143 17.33 ± 0.33c 15.00 ± 0.00b 17.66 ± 0.66c 18.66 ± 0.66b 15.66 ± 0.33b K. pneumonia ATCC700603a 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c E. faecilis ATCC700802a 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c S. enterica ATCC700408a 12.00 ± 0.00d 15.33 ± 0.33b 9.66 ± 0.88d 16.00 ± 0.00b 15.66 ± 0.66b Data is expressed as mean ± SEM, n = 9.Within a row, mean values with lowercase letters are significantly different (P < 0.05). ..
Isolation:Article Title: Lactic acid bacteria isolated from fermented flour of finger millet, its probiotic attributes and bioactive properties
Article Snippet: .. Tanaka et al. (1999) stated that BSH activity is high in genus Bifidobacterium compared to Lactobacillus and further suggested that high BSH activity is commonly present in strains isolated from gastrointestinal track of mammals, when Table 8 Anti-bacterial activity of intracellular cell free extracts of isolated lactic acid bacteria Test pathogens Mean diameter of the zones of inhibition (in mm) R17 RV02 RV19 RV28 O24 E. coli ATCC 2592 15.33 ± 0.33b 16.67 ± 1.45b 15.33 ± 0.33b 17.33 ± 0.33b 15.33 ± 0.33b K. pneumonia ATCC 35594 15.33 ± 0.33c 14.66 ± 0.33c 15.00 ± 0.00c 18.33 ± 0.33b 18.33 ± 0.33b S. aureus ATCC 6571 10.33 ± 0.33d 13.33 ± 0.33c 12.66 ± 0.33c 13.33 ± 0.33c 13.00 ± 0.57c S. sanguinis ATCC 10556 0.00 ± 0.00b 0.00 ± 0.00b 0.00 ± 0.00b 0.00 ± 0.00b 0.00 ± 0.00b S. salvarius ATCC 13419 14.66 ± 0.33d 16.00 ± 0.00c 16.00 ± 0.00c 10.66 ± 0.33e 14.00 ± 0.00d S. flexenari ATCC 12022 14.33 ± 0.33b 15.66 ± 0.33b 16.00 ± 0.00b 12.33 ± 0.66c 15.66 ± 0.33b E. faecilis ATCC 49532 8.33 ± 0.33d 0.00 ± 0.00e 0.00 ± 0.00e 0.00 ± 0.00c 12.66 ± 0.33e A. baumani ATCC 17978 0.00 ± 0.00g 15.33 ± 0.33c 11.33 ± 0.33f 13.00 ± 0.00e 14.33 ± 0.33d S. mutans ATCC 25175 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c S. pyogenes ATCC 700294 0.00 ± 0.00e 12.00 ± 0.00c 12.33 ± 0.33c 15.00 ± 0.57b 13.66 ± 0.33b,c E. coli ATCC35218a 15.33 ± 0.33c 16.00 ± 0.00c 18.33 ± 0.33b 16.66 ± 0.33b,c 15.33 ± 0.33c S. aureusa NCTC13143 17.33 ± 0.33c 15.00 ± 0.00b 17.66 ± 0.66c 18.66 ± 0.66b 15.66 ± 0.33b K. pneumonia ATCC700603a 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c E. faecilis ATCC700802a 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c S. enterica ATCC700408a 12.00 ± 0.00d 15.33 ± 0.33b 9.66 ± 0.88d 16.00 ± 0.00b 15.66 ± 0.66b Data is expressed as mean ± SEM, n = 9.Within a row, mean values with lowercase letters are significantly different (P < 0.05). ..
Bacteria:Article Title: Lactic acid bacteria isolated from fermented flour of finger millet, its probiotic attributes and bioactive properties
Article Snippet: .. Tanaka et al. (1999) stated that BSH activity is high in genus Bifidobacterium compared to Lactobacillus and further suggested that high BSH activity is commonly present in strains isolated from gastrointestinal track of mammals, when Table 8 Anti-bacterial activity of intracellular cell free extracts of isolated lactic acid bacteria Test pathogens Mean diameter of the zones of inhibition (in mm) R17 RV02 RV19 RV28 O24 E. coli ATCC 2592 15.33 ± 0.33b 16.67 ± 1.45b 15.33 ± 0.33b 17.33 ± 0.33b 15.33 ± 0.33b K. pneumonia ATCC 35594 15.33 ± 0.33c 14.66 ± 0.33c 15.00 ± 0.00c 18.33 ± 0.33b 18.33 ± 0.33b S. aureus ATCC 6571 10.33 ± 0.33d 13.33 ± 0.33c 12.66 ± 0.33c 13.33 ± 0.33c 13.00 ± 0.57c S. sanguinis ATCC 10556 0.00 ± 0.00b 0.00 ± 0.00b 0.00 ± 0.00b 0.00 ± 0.00b 0.00 ± 0.00b S. salvarius ATCC 13419 14.66 ± 0.33d 16.00 ± 0.00c 16.00 ± 0.00c 10.66 ± 0.33e 14.00 ± 0.00d S. flexenari ATCC 12022 14.33 ± 0.33b 15.66 ± 0.33b 16.00 ± 0.00b 12.33 ± 0.66c 15.66 ± 0.33b E. faecilis ATCC 49532 8.33 ± 0.33d 0.00 ± 0.00e 0.00 ± 0.00e 0.00 ± 0.00c 12.66 ± 0.33e A. baumani ATCC 17978 0.00 ± 0.00g 15.33 ± 0.33c 11.33 ± 0.33f 13.00 ± 0.00e 14.33 ± 0.33d S. mutans ATCC 25175 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c S. pyogenes ATCC 700294 0.00 ± 0.00e 12.00 ± 0.00c 12.33 ± 0.33c 15.00 ± 0.57b 13.66 ± 0.33b,c E. coli ATCC35218a 15.33 ± 0.33c 16.00 ± 0.00c 18.33 ± 0.33b 16.66 ± 0.33b,c 15.33 ± 0.33c S. aureusa NCTC13143 17.33 ± 0.33c 15.00 ± 0.00b 17.66 ± 0.66c 18.66 ± 0.66b 15.66 ± 0.33b K. pneumonia ATCC700603a 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c E. faecilis ATCC700802a 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c S. enterica ATCC700408a 12.00 ± 0.00d 15.33 ± 0.33b 9.66 ± 0.88d 16.00 ± 0.00b 15.66 ± 0.66b Data is expressed as mean ± SEM, n = 9.Within a row, mean values with lowercase letters are significantly different (P < 0.05). ..
Article Title: <p>Potential Allylpyrocatechol Derivatives as Antibacterial Agent Against Oral Pathogen of<em> S. sanguinis</em> ATCC 10,556 and as Inhibitor of MurA Enzymes: in vitro and in silico Study</p>
Article Snippet: .. S. sanguinis ATCC 10,556 and as Inhibitor of MurA Enzymes: in vitro and in silico Study This article was published in the following Dove Press journal: Drug Design, Development and Therapy Dikdik Kurnia Geofanny Sarah Hutabarat Devi Windaryanti Tati Herlina Yetty Herdiyati Mieke Hemiawati Satari 1Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Sumedang, Indonesia; 2Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Indonesia; 3Department of Oral Biology, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Indonesia Background: Streptococcus sanguinis is Gram-positive bacteria that contribute to caries. ..
Inhibition:Article Title: Lactic acid bacteria isolated from fermented flour of finger millet, its probiotic attributes and bioactive properties
Article Snippet: .. Tanaka et al. (1999) stated that BSH activity is high in genus Bifidobacterium compared to Lactobacillus and further suggested that high BSH activity is commonly present in strains isolated from gastrointestinal track of mammals, when Table 8 Anti-bacterial activity of intracellular cell free extracts of isolated lactic acid bacteria Test pathogens Mean diameter of the zones of inhibition (in mm) R17 RV02 RV19 RV28 O24 E. coli ATCC 2592 15.33 ± 0.33b 16.67 ± 1.45b 15.33 ± 0.33b 17.33 ± 0.33b 15.33 ± 0.33b K. pneumonia ATCC 35594 15.33 ± 0.33c 14.66 ± 0.33c 15.00 ± 0.00c 18.33 ± 0.33b 18.33 ± 0.33b S. aureus ATCC 6571 10.33 ± 0.33d 13.33 ± 0.33c 12.66 ± 0.33c 13.33 ± 0.33c 13.00 ± 0.57c S. sanguinis ATCC 10556 0.00 ± 0.00b 0.00 ± 0.00b 0.00 ± 0.00b 0.00 ± 0.00b 0.00 ± 0.00b S. salvarius ATCC 13419 14.66 ± 0.33d 16.00 ± 0.00c 16.00 ± 0.00c 10.66 ± 0.33e 14.00 ± 0.00d S. flexenari ATCC 12022 14.33 ± 0.33b 15.66 ± 0.33b 16.00 ± 0.00b 12.33 ± 0.66c 15.66 ± 0.33b E. faecilis ATCC 49532 8.33 ± 0.33d 0.00 ± 0.00e 0.00 ± 0.00e 0.00 ± 0.00c 12.66 ± 0.33e A. baumani ATCC 17978 0.00 ± 0.00g 15.33 ± 0.33c 11.33 ± 0.33f 13.00 ± 0.00e 14.33 ± 0.33d S. mutans ATCC 25175 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c S. pyogenes ATCC 700294 0.00 ± 0.00e 12.00 ± 0.00c 12.33 ± 0.33c 15.00 ± 0.57b 13.66 ± 0.33b,c E. coli ATCC35218a 15.33 ± 0.33c 16.00 ± 0.00c 18.33 ± 0.33b 16.66 ± 0.33b,c 15.33 ± 0.33c S. aureusa NCTC13143 17.33 ± 0.33c 15.00 ± 0.00b 17.66 ± 0.66c 18.66 ± 0.66b 15.66 ± 0.33b K. pneumonia ATCC700603a 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c E. faecilis ATCC700802a 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c 0.00 ± 0.00c S. enterica ATCC700408a 12.00 ± 0.00d 15.33 ± 0.33b 9.66 ± 0.88d 16.00 ± 0.00b 15.66 ± 0.66b Data is expressed as mean ± SEM, n = 9.Within a row, mean values with lowercase letters are significantly different (P < 0.05). ..
Quantitative RT-PCR:Article Title: Key virulence genes associated with Streptococcus mutans biofilm formation: a systematic review
Article Snippet: .. ( ) , Natural flavone luteolin , S. mutans ATCC 25,175, S. sanguinis ATCC 10,556, S. gordonii ATCC 10,558, S. mitis ATCC 49,456, S. oralis ATCC 35,037 , Brain Heart Infusion (BHI) broth , spaP , , , qRT-PCR of S. mutans biofilms treated with 25 μg/ml luteolin for 24 h was performed, with RNA extracted using TRIzol and cDNA synthesized using the GoScriptTM Reverse Transcriptase system. Gene expression was normalized to 16S rRNA, and mRNA levels were quantified using the FastStart Universal SYBR Green Master and analyzed with the 2− ΔΔ Ct method. , Luteolin downregulated key virulence genes (gbpC, spaP, gtfBCD, ftf), which led to reduced production of surface adhesins and extracellular polysaccharides (EPS), and interfered with glucosyltransferases (Gtfs), decreasing the synthesis of water-insoluble glucans. ..
Synthesized:Article Title: Key virulence genes associated with Streptococcus mutans biofilm formation: a systematic review
Article Snippet: .. ( ) , Natural flavone luteolin , S. mutans ATCC 25,175, S. sanguinis ATCC 10,556, S. gordonii ATCC 10,558, S. mitis ATCC 49,456, S. oralis ATCC 35,037 , Brain Heart Infusion (BHI) broth , spaP , , , qRT-PCR of S. mutans biofilms treated with 25 μg/ml luteolin for 24 h was performed, with RNA extracted using TRIzol and cDNA synthesized using the GoScriptTM Reverse Transcriptase system. Gene expression was normalized to 16S rRNA, and mRNA levels were quantified using the FastStart Universal SYBR Green Master and analyzed with the 2− ΔΔ Ct method. , Luteolin downregulated key virulence genes (gbpC, spaP, gtfBCD, ftf), which led to reduced production of surface adhesins and extracellular polysaccharides (EPS), and interfered with glucosyltransferases (Gtfs), decreasing the synthesis of water-insoluble glucans. ..
Reverse Transcription:Article Title: Key virulence genes associated with Streptococcus mutans biofilm formation: a systematic review
Article Snippet: .. ( ) , Natural flavone luteolin , S. mutans ATCC 25,175, S. sanguinis ATCC 10,556, S. gordonii ATCC 10,558, S. mitis ATCC 49,456, S. oralis ATCC 35,037 , Brain Heart Infusion (BHI) broth , spaP , , , qRT-PCR of S. mutans biofilms treated with 25 μg/ml luteolin for 24 h was performed, with RNA extracted using TRIzol and cDNA synthesized using the GoScriptTM Reverse Transcriptase system. Gene expression was normalized to 16S rRNA, and mRNA levels were quantified using the FastStart Universal SYBR Green Master and analyzed with the 2− ΔΔ Ct method. , Luteolin downregulated key virulence genes (gbpC, spaP, gtfBCD, ftf), which led to reduced production of surface adhesins and extracellular polysaccharides (EPS), and interfered with glucosyltransferases (Gtfs), decreasing the synthesis of water-insoluble glucans. ..
Gene Expression:Article Title: Key virulence genes associated with Streptococcus mutans biofilm formation: a systematic review
Article Snippet: .. ( ) , Natural flavone luteolin , S. mutans ATCC 25,175, S. sanguinis ATCC 10,556, S. gordonii ATCC 10,558, S. mitis ATCC 49,456, S. oralis ATCC 35,037 , Brain Heart Infusion (BHI) broth , spaP , , , qRT-PCR of S. mutans biofilms treated with 25 μg/ml luteolin for 24 h was performed, with RNA extracted using TRIzol and cDNA synthesized using the GoScriptTM Reverse Transcriptase system. Gene expression was normalized to 16S rRNA, and mRNA levels were quantified using the FastStart Universal SYBR Green Master and analyzed with the 2− ΔΔ Ct method. , Luteolin downregulated key virulence genes (gbpC, spaP, gtfBCD, ftf), which led to reduced production of surface adhesins and extracellular polysaccharides (EPS), and interfered with glucosyltransferases (Gtfs), decreasing the synthesis of water-insoluble glucans. ..
SYBR Green Assay:Article Title: Key virulence genes associated with Streptococcus mutans biofilm formation: a systematic review
Article Snippet: .. ( ) , Natural flavone luteolin , S. mutans ATCC 25,175, S. sanguinis ATCC 10,556, S. gordonii ATCC 10,558, S. mitis ATCC 49,456, S. oralis ATCC 35,037 , Brain Heart Infusion (BHI) broth , spaP , , , qRT-PCR of S. mutans biofilms treated with 25 μg/ml luteolin for 24 h was performed, with RNA extracted using TRIzol and cDNA synthesized using the GoScriptTM Reverse Transcriptase system. Gene expression was normalized to 16S rRNA, and mRNA levels were quantified using the FastStart Universal SYBR Green Master and analyzed with the 2− ΔΔ Ct method. , Luteolin downregulated key virulence genes (gbpC, spaP, gtfBCD, ftf), which led to reduced production of surface adhesins and extracellular polysaccharides (EPS), and interfered with glucosyltransferases (Gtfs), decreasing the synthesis of water-insoluble glucans. ..
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